Hotspot

ycf2-rpl10

IRb · 94728-95960 · 1233 bp

Back to Convallaria

Pi 0.00703

MarkerSeek score 51.30

Primer available yes

Label ycf2-rpl10

Feature Metadata

label_name: ycf2-rpl10
feature_type: igs
region: IRb
length_bp: 1233
variable_sites: 13
pi: 0.00703
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-rpl10_p1	AAAGAGATGGCTTTTCCCGG	CTCCATGGACCAAGATCTGC	50.0	55.0	58.2	58.0	1407-1429 bp	1.0	79.5
ycf2-rpl10_p2	ATGGCTTTTCCCGGATGAAA	CTCCATGGACCAAGATCTGC	45.0	55.0	57.8	58.0	1401-1423 bp	1.0	79.0
ycf2-rpl10_p3	TGGCTTTTCCCGGATGAAAT	CTCCATGGACCAAGATCTGC	45.0	55.0	57.8	58.0	1400-1422 bp	1.0	79.0
ycf2-rpl10_p4	GATGGCTTTTCCCGGATGAA	CTCCATGGACCAAGATCTGC	50.0	55.0	58.2	58.0	1402-1424 bp	1.0	78.9
ycf2-rpl10_p5	AAAGAGATGGCTTTTCCCGG	CTTCTTGGGACTCCATGGAC	50.0	55.0	58.2	57.6	1417-1439 bp	1.0	76.5