Hotspot

psbE-petL

LSC · 65922-67237 · 1316 bp

Back to Convallaria

Pi 0.00253

MarkerSeek score 66.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1316
variable_sites: 5
pi: 0.00253
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCAGCCAATAGAAAACCGA	50.0	45.0	57.9	57.5	1397-1462 bp	1.0	79.6
psbE-petL_p2	ACTGACGAATAACCAACCCG	AGCAGCCAATAGAAAACCGA	50.0	45.0	57.9	57.5	1410-1475 bp	1.0	79.2
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGCAGCCAATAGAAAACCGA	45.0	45.0	58.2	57.5	1406-1471 bp	1.0	78.6
psbE-petL_p4	CGAATAACCAACCCGCAATG	AGCAGCCAATAGAAAACCGA	50.0	45.0	57.8	57.5	1405-1470 bp	1.0	78.4
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AGCAGCCAATAGAAAACCGA	50.0	45.0	58.2	57.5	1399-1464 bp	1.0	78.1