Hotspot

petA-psbJ

LSC · 64054-65152 · 1099 bp

Back to Convallaria

Pi 0.00667

MarkerSeek score 50.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1099
variable_sites: 11
pi: 0.00667
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCCGTTATTTTGGCAC	CCGGATTGGGTTCATCTCTG	50.0	55.0	58.1	58.0	1206-1207 bp	1.0	79.8
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	CCGGATTGGGTTCATCTCTG	50.0	55.0	57.8	58.0	1223-1224 bp	1.0	79.2
petA-psbJ_p3	GGCATCCGTTATTTTGGCAC	TCCGGATTGGGTTCATCTCT	50.0	50.0	58.1	58.1	1207-1208 bp	1.0	79.2
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	TCCGGATTGGGTTCATCTCT	50.0	50.0	57.8	58.1	1224-1225 bp	1.0	78.6
petA-psbJ_p5	TGTTCTTCTTGGCATCCGTT	CCGGATTGGGTTCATCTCTG	45.0	55.0	57.7	58.0	1216-1217 bp	1.0	78.3