Hotspot

psaA-ycf3

LSC · 42168-42806 · 639 bp

Back to Convallaria

Pi 0

MarkerSeek score 60.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 639
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	737-745 bp	1.0	78.9
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	741-749 bp	1.0	78.7
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	TGAAGATCACGAGGCGTTTC	50.0	50.0	57.9	58.3	736-744 bp	1.0	77.9
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	TCACGAGGCGTTTCGAATAA	50.0	45.0	57.9	57.6	730-738 bp	1.0	77.4
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	782-790 bp	1.0	77.3