Hotspot

trnT-GGU-psbD

LSC · 32453-33214 · 762 bp

Back to Convallaria

Pi 0.00525

MarkerSeek score 62.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 762
variable_sites: 6
pi: 0.00525
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	922-925 bp	1.0	79.1
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	925-928 bp	1.0	78.9
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	899-902 bp	1.0	77.4
trnT-GGU-psbD_p4	GGCCCTTTTAACTCAGTGGT	GGACCAGCCTACAAAAACGA	50.0	50.0	57.7	58.1	937-940 bp	1.0	77.3
trnT-GGU-psbD_p5	TGGTAGAGTAACGCCATGGT	GGACCAGCCTACAAAAACGA	50.0	50.0	58.4	58.1	921-924 bp	1.0	76.1