Hotspot

ycf89

Genome · 127743-128705 · 963 bp

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Pi 0.184

MarkerSeek score 78.80

Primer available yes

Label ycf89

Feature Metadata

label_name: ycf89
feature_type: gene
region: Genome
length_bp: 963
variable_sites: 177
pi: 0.184
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf89_p1	TCTCGTACGACTTGCATGTG	AGGATTTGAACCTAGGGCAC	50.0	50.0	58.0	57.2	1724-1734 bp	1.0	83.0
ycf89_p2	CATTGCGGAAAATTCCCCAC	AGGATTTGAACCTAGGGCAC	50.0	50.0	58.0	57.2	2000-2011 bp	1.0	82.9
ycf89_p3	TATTGCTCCGTCAGGCTTTC	AGGATTTGAACCTAGGGCAC	50.0	50.0	58.0	57.2	2023-2034 bp	1.0	82.8
ycf89_p4	ATTGCGGAAAATTCCCCACT	AGGATTTGAACCTAGGGCAC	45.0	50.0	58.1	57.2	1999-2010 bp	1.0	82.5
ycf89_p5	CAACCCATAGGCAGTCATCC	AGGATTTGAACCTAGGGCAC	55.0	50.0	58.0	57.2	2063 bp	0.5	53.3