Hotspot

rpl2

Genome · 92148-92975 · 828 bp

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Pi 0.128

MarkerSeek score 79.80

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: Genome
length_bp: 828
variable_sites: 106
pi: 0.128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	GGTGGGGTGATATTTGGACC	ATAGTCGAGCTACGTGACCA	55.0	50.0	57.9	58.0	1699 bp	0.5	59.2
rpl2_p2	GGTGGGGTGATATTTGGACC	GAGCTACGTGACCAAGTTGT	55.0	50.0	57.9	57.8	1693 bp	0.5	58.3
rpl2_p3	GGTGGGGTGATATTTGGACC	TAGTCGAGCTACGTGACCAA	55.0	50.0	57.9	58.2	1698 bp	0.5	58.0
rpl2_p4	GGTGGTGGGGTGATATTTGG	ATAGTCGAGCTACGTGACCA	55.0	50.0	58.2	58.0	1702 bp	0.5	57.9
rpl2_p5	GGTGGTGGGGTGATATTTGG	GAGCTACGTGACCAAGTTGT	55.0	50.0	58.2	57.8	1696 bp	0.5	57.0