Hotspot

clpC

Genome · 106711-109383 · 2673 bp

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Pi 0.254

MarkerSeek score 78.00

Primer available yes

Label clpC

Feature Metadata

label_name: clpC
feature_type: gene
region: Genome
length_bp: 2673
variable_sites: 668
pi: 0.254
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpC_p1	GGCACAAACGAAACGTGAAT	AGGTGTACAGTCAGGACGAT	45.0	50.0	57.9	57.8	2969 bp	0.5	57.3
clpC_p2	GGCACAAACGAAACGTGAAT	AGCTGGTCGAATGGAAAAGA	45.0	45.0	57.9	57.1	2939 bp	0.5	52.2
clpC_p3	GGCACAAACGAAACGTGAAT	TAGGTGTACAGTCAGGACGA	45.0	50.0	57.9	57.1	2970 bp	0.5	52.2
clpC_p4	GGCACAAACGAAACGTGAAT	GGTGTACAGTCAGGACGATT	45.0	50.0	57.9	57.0	2968 bp	0.5	50.8
clpC_p5	GGCACAAACGAAACGTGAAT	AGCTGGTCGAATGGAAAAGAA	45.0	42.9	57.9	57.9	2939 bp	0.5	50.0