Hotspot

dnaK-rpl3

Genome · 90179-90500 · 322 bp

Back to Conticribra

Pi 0.248
MarkerSeek score 81.20
Primer available yes
Label dnaK-rpl3

Feature Metadata

label_name
dnaK-rpl3
feature_type
igs
region
Genome
length_bp
322
variable_sites
76
pi
0.248
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
dnaK-rpl3_p1 TGTTGTTCCTAAATCAATTCCAACT TGGGTCATTCCAATTTTGTTACC 32.0 39.1 57.4 57.5 399-400 bp 1.0 51.0
dnaK-rpl3_p2 TGTTGTTCCTAAATCAATTCCAACT GGGTCATTCCAATTTTGTTACCT 32.0 39.1 57.4 57.3 398-399 bp 1.0 51.0
dnaK-rpl3_p3 TGTTGTTCCTAAATCAATTCCAAC TGGGTCATTCCAATTTTGTTACC 33.3 39.1 56.1 57.5 399-400 bp 1.0 51.0
dnaK-rpl3_p4 GCAACTACAGAATTTGTTGT TGGGTCATTCCAATTTTGTTACC 35.0 39.1 52.3 57.5 413-414 bp 1.0 50.9
dnaK-rpl3_p5 GCAACTACAGAATTTGTTGT GGGTCATTCCAATTTTGTTACCT 35.0 39.1 52.3 57.3 412-413 bp 1.0 50.9