Hotspot

trnP-UGG-ycf89

Genome · 66197-66546 · 350 bp

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Pi 0.266

MarkerSeek score 77.50

Primer available yes

Label trnP-UGG-ycf89

Feature Metadata

label_name: trnP-UGG-ycf89
feature_type: igs
region: Genome
length_bp: 350
variable_sites: 90
pi: 0.266
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-ycf89_p1	GAAGATGGAGTCATGCAGCA	ATTCCAGGAGCATCGGGATA	50.0	50.0	58.0	58.0	1099 bp	0.5	59.5
trnP-UGG-ycf89_p2	GATGGAGTCATGCAGCAGAA	ATTCCAGGAGCATCGGGATA	50.0	50.0	58.0	58.0	1096 bp	0.5	59.5
trnP-UGG-ycf89_p3	AAGATGGAGTCATGCAGCAG	ATTCCAGGAGCATCGGGATA	50.0	50.0	58.0	58.0	1098 bp	0.5	59.4
trnP-UGG-ycf89_p4	GAAGATGGAGTCATGCAGCA	GCAAAGACCCATGGATACGT	50.0	50.0	58.0	58.0	1513 bp	0.5	59.4
trnP-UGG-ycf89_p5	GATGGAGTCATGCAGCAGAA	GCAAAGACCCATGGATACGT	50.0	50.0	58.0	58.0	1510 bp	0.5	59.4