Hotspot

rpoB-rps20

Genome · 39768-40255 · 488 bp

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Pi 0.273

MarkerSeek score 79.60

Primer available yes

Label rpoB-rps20

Feature Metadata

label_name: rpoB-rps20
feature_type: igs
region: Genome
length_bp: 488
variable_sites: 116
pi: 0.273
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-rps20_p1	AGGAATAACCAGTTGTGCCG	AGAAACACCAGCAGAACGTT	50.0	45.0	58.2	58.0	1321 bp	0.5	58.3
rpoB-rps20_p2	GGAATAACCAGTTGTGCCGA	AGAAACACCAGCAGAACGTT	50.0	45.0	58.2	58.0	1320 bp	0.5	58.2
rpoB-rps20_p3	TGCCGAGTAATTTGCGGTAT	AGAAACACCAGCAGAACGTT	45.0	45.0	57.7	58.0	1306 bp	0.5	57.1
rpoB-rps20_p4	GTTGTGCCGAGTAATTTGCG	AGAAACACCAGCAGAACGTT	50.0	45.0	58.4	58.0	1310 bp	0.5	56.5
rpoB-rps20_p5	ACCAGTTGTGCCGAGTAATT	AGAAACACCAGCAGAACGTT	45.0	45.0	57.4	58.0	1314 bp	0.5	55.2