Hotspot

psaB-petF

Genome · 4727-5908 · 1182 bp

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Pi 0.286

MarkerSeek score 64.70

Primer available yes

Label psaB-petF

Feature Metadata

label_name: psaB-petF
feature_type: igs
region: Genome
length_bp: 1182
variable_sites: 75
pi: 0.286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.22
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB-petF_p1	GAGCTTTAGATGCACGTGGA	AGATGCAGCAGAAGAAGCAG	50.0	50.0	58.0	58.3	1995 bp	0.5	57.8
psaB-petF_p2	AGGAGCTTTAGATGCACGTG	AGATGCAGCAGAAGAAGCAG	50.0	50.0	58.0	58.3	1997 bp	0.5	57.8
psaB-petF_p3	GGCACATGAACGTACTCCAT	AGATGCAGCAGAAGAAGCAG	50.0	50.0	58.0	58.3	1565 bp	0.5	57.7
psaB-petF_p4	AAAAATCGGCCCAGGTGATT	AGATGCAGCAGAAGAAGCAG	45.0	50.0	58.1	58.3	2075 bp	0.5	57.4
psaB-petF_p5	AAAATCGGCCCAGGTGATTT	AGATGCAGCAGAAGAAGCAG	45.0	50.0	58.1	58.3	2074 bp	0.5	57.4