Hotspot

rpl22

LSC · 85029-85568 · 540 bp

Back to Consolida

Pi 0.00988

MarkerSeek score 49.80

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 540
variable_sites: 8
pi: 0.00988
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TGGTTGTGCAAACCAAACAG	GGGACATGCGAGAAACGATA	45.0	50.0	57.6	57.8	690 bp	1.0	76.2
rpl22_p2	TGGTTGTGCAAACCAAACAG	TCTGAATTTCCGGGGACATG	45.0	50.0	57.6	57.6	702 bp	1.0	74.6
rpl22_p3	TGGTTGTGCAAACCAAACAG	CGTTTATTGGTGGGAGGTGA	45.0	50.0	57.6	57.5	627 bp	1.0	74.1
rpl22_p4	TGGTTGTGCAAACCAAACAG	TCGTTTATTGGTGGGAGGTG	45.0	50.0	57.6	57.5	628 bp	1.0	74.1
rpl22_p5	GGTTGTGCAAACCAAACAGA	GGGACATGCGAGAAACGATA	45.0	50.0	57.3	57.8	689 bp	1.0	73.9