Hotspot

ccsA-ndhD

SSC · 116951-117266 · 316 bp

Back to Consolida

Pi 0.0422

MarkerSeek score 54.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 316
variable_sites: 20
pi: 0.0422
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCTATGCAGCTCTTCTGTGT	CACTTGCATTGCCTGGAATG	50.0	50.0	58.0	58.0	1200-1205 bp	1.0	82.1
ccsA-ndhD_p2	GCTATGCAGCTCTTCTGTGT	ACTTGCATTGCCTGGAATGA	50.0	45.0	58.0	58.1	1199-1204 bp	1.0	81.6
ccsA-ndhD_p3	GCTATGCAGCTCTTCTGTGT	TCACTTGCATTGCCTGGAAT	50.0	45.0	58.0	58.1	1201-1206 bp	1.0	81.6
ccsA-ndhD_p4	GCTATGCAGCTCTTCTGTGT	TTGCATTGCCTGGAATGAGT	50.0	45.0	58.0	58.1	1197-1202 bp	1.0	81.6
ccsA-ndhD_p5	GCTATGCAGCTCTTCTGTGT	TGGTGCTGCACTTTTCTTCT	50.0	45.0	58.0	57.9	1331-1336 bp	1.0	81.5