Hotspot

psbE-petL

LSC · 65874-66895 · 1022 bp

Back to Consolida

Pi 0.00783

MarkerSeek score 43.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1022
variable_sites: 12
pi: 0.00783
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGTCGCCAGTAGAAAACCG	50.0	50.0	57.9	58.2	1152-1169 bp	1.0	80.1
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GTCGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.2	1150-1167 bp	1.0	80.0
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AAGTCGCCAGTAGAAAACCG	45.0	50.0	58.2	58.2	1161-1178 bp	1.0	79.1
psbE-petL_p4	ACGAATAACCAACCCGCAAT	GTCGCCAGTAGAAAACCGAA	45.0	50.0	58.2	58.2	1159-1176 bp	1.0	79.0
psbE-petL_p5	TGCTGACGAATAACCAACCC	AAGTCGCCAGTAGAAAACCG	50.0	50.0	58.2	58.2	1166-1183 bp	1.0	79.0