Hotspot

rbcL-accD

LSC · 58532-59260 · 729 bp

Back to Consolida

Pi 0.00732

MarkerSeek score 61.70

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 729
variable_sites: 8
pi: 0.00732
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TGCAAATGGAGCCTTGAACT	ATTTACTTAGCCCGCACCTG	45.0	50.0	58.0	58.0	858-883 bp	1.0	83.7
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	ATTTACTTAGCCCGCACCTG	50.0	50.0	57.9	58.0	834-859 bp	1.0	82.9
rbcL-accD_p3	TGCAAATGGAGCCTTGAACT	TAGCGATTTACTTAGCCCGC	45.0	50.0	58.0	57.9	863-888 bp	1.0	82.8
rbcL-accD_p4	GCTGCTTGTGAGGTATGGAA	TAGCGATTTACTTAGCCCGC	50.0	50.0	57.9	57.9	839-864 bp	1.0	82.0
rbcL-accD_p5	TGCAAATGGAGCCTTGAACT	GATTTACTTAGCCCGCACCT	45.0	50.0	58.0	57.7	859-884 bp	1.0	81.4