Hotspot

trnF-GAA-ndhJ

LSC · 49844-50517 · 674 bp

Back to Consolida

Pi 0.00989

MarkerSeek score 51.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 674
variable_sites: 10
pi: 0.00989
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGGTGGCC	50.0	55.0	58.1	58.2	783-803 bp	1.0	79.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCATGAAACGT	50.0	45.0	58.1	58.3	815-835 bp	1.0	78.8
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TCATCCACGCATGAAACGTA	50.0	45.0	58.1	57.6	814-834 bp	1.0	78.0
trnF-GAA-ndhJ_p4	GTCGGGAAAAGTCGGGATAG	GAAAGTTGGATAGGGTGGCC	55.0	55.0	57.8	58.2	832-852 bp	1.0	77.7
trnF-GAA-ndhJ_p5	GGGAAAAGTCGGGATAGCTC	GAAAGTTGGATAGGGTGGCC	55.0	55.0	57.8	58.2	829-849 bp	1.0	77.5