Hotspot

trnC-GCA-petN

LSC · 27666-28529 · 864 bp

Back to Consolida

Pi 0.00617

MarkerSeek score 58.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 864
variable_sites: 8
pi: 0.00617
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	927 bp	1.0	80.5
trnC-GCA-petN_p2	CACCAGCACCAGGAAGATTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	1009 bp	1.0	80.4
trnC-GCA-petN_p3	CAGCACCAGGAAGATTTCGA	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	57.9	58.0	1006 bp	1.0	80.3
trnC-GCA-petN_p4	ACCAGCACCAGGAAGATTTC	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	57.8	58.0	1008 bp	1.0	79.4
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	921 bp	1.0	79.0