Hotspot

trnS-GCU-trnG-UCC

LSC · 7793-8497 · 705 bp

Back to Consolida

Pi 0.00662
MarkerSeek score 51.40
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
705
variable_sites
7
pi
0.00662
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA AACCGAAAGACCCCTTAACT 50.0 45.0 58.0 56.4 812-829 bp 1.0 68.1
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC AACCGAAAGACCCCTTAACT 50.0 45.0 58.0 56.4 811-828 bp 1.0 68.1
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA AACCGAAAGACCCCTTAACT 50.0 45.0 58.2 56.4 866-883 bp 1.0 66.9
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA AAACCGAAAGACCCCTTAACT 50.0 42.9 58.0 57.1 813-830 bp 1.0 66.3
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC AAACCGAAAGACCCCTTAACT 50.0 42.9 58.0 57.1 812-829 bp 1.0 66.3