Hotspot

rps16-trnQ-UUG

LSC · 5132-6419 · 1288 bp

Back to Consolida

Pi 0.0109

MarkerSeek score 41.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1288
variable_sites: 21
pi: 0.0109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTGTCAATCGAATCCACGGA	GAAATTGAAATGGGGCGTGG	50.0	50.0	58.0	58.0	2107-2469 bp	1.0	82.7
rps16-trnQ-UUG_p2	TCAATCGAATCCACGGAACC	GAAATTGAAATGGGGCGTGG	50.0	50.0	58.0	58.0	2104-2466 bp	1.0	82.6
rps16-trnQ-UUG_p3	TGTGTTGGATTGGCACTTGA	GAAATTGAAATGGGGCGTGG	45.0	50.0	57.9	58.0	1930-2289 bp	1.0	82.3
rps16-trnQ-UUG_p4	GTGTCAATCGAATCCACGGA	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	2608-2976 bp	1.0	81.9
rps16-trnQ-UUG_p5	TCAATCGAATCCACGGAACC	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	2605-2973 bp	1.0	81.8