Hotspot

trnK-UUU-rps16

LSC · 4313-4916 · 604 bp

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Pi 0.0177
MarkerSeek score 52.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
604
variable_sites
16
pi
0.0177
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GAGTTCAACCAACCTACGGG 50.0 55.0 58.2 58.2 716-720 bp 1.0 80.2
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT GGAGTTCAACCAACCTACGG 50.0 55.0 58.2 58.2 717-721 bp 1.0 80.2
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT GAGTTCAACCAACCTACGGG 50.0 55.0 58.2 58.2 715-719 bp 1.0 80.2
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GGAGTTCAACCAACCTACGG 50.0 55.0 58.2 58.2 716-720 bp 1.0 80.2
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TTCAACCAACCTACGGGAAC 50.0 50.0 58.2 57.7 712-716 bp 1.0 79.7