Hotspot

rps8

LSC · 3809-4213 · 405 bp

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Pi 0.00494

MarkerSeek score 57.50

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 2
pi: 0.00494
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	AAATTCGGAGACACCACTGA	GGGACCAACTAATAGGGGGA	45.0	55.0	56.8	57.8	526 bp	1.0	68.9
rps8_p2	TCGGAGACACCACTGATTTT	GGGACCAACTAATAGGGGGA	45.0	55.0	56.8	57.8	522 bp	1.0	68.9
rps8_p3	TTCGGAGACACCACTGATTT	GGGACCAACTAATAGGGGGA	45.0	55.0	56.8	57.8	523 bp	1.0	68.9
rps8_p4	TCGGAGACACCACTGATTTT	AGGGACCAACTAATAGGGGG	45.0	55.0	56.8	57.8	523 bp	1.0	68.9
rps8_p5	TTCGGAGACACCACTGATTT	AGGGACCAACTAATAGGGGG	45.0	55.0	56.8	57.8	524 bp	1.0	68.9