Hotspot

rpoC2

LSC · 20579-24643 · 4065 bp

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Pi 0.000492

MarkerSeek score 54.20

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4065
variable_sites: 2
pi: 0.000492
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CGCCTCTTTGCCAATAAATCA	ACTGATGTGTACCATGCACC	42.9	50.0	57.2	57.9	335 bp	1.0	65.1
rpoC2_p2	CGCCTCTTTGCCAATAAATCA	ATCAGGAAGGCCGTACAGAT	42.9	50.0	57.2	58.2	258 bp	1.0	64.4
rpoC2_p3	CGCCTCTTTGCCAATAAATCA	ACCATGCACCCGAATTTACA	42.9	45.0	57.2	57.8	325 bp	1.0	64.3
rpoC2_p4	CGCCTCTTTGCCAATAAATCA	TGTACCATGCACCCGAATTT	42.9	45.0	57.2	57.8	328 bp	1.0	64.3
rpoC2_p5	CGCCTCTTTGCCAATAAATCA	GGGGAAATGCACTGGAGTAC	42.9	55.0	57.2	58.2	353 bp	1.0	64.0