Hotspot

ndhF-rpl32

IRb · 110413-111671 · 1259 bp

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Pi 0.000794

MarkerSeek score 59.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: IRb
length_bp: 1259
variable_sites: 1
pi: 0.000794
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GAAGCGGAGTGAAGGGTATG	TTAACGCTGCCCAATATCCC	55.0	50.0	58.1	58.0	1396 bp	1.0	79.4
ndhF-rpl32_p2	GCGGAGTGAAGGGTATGATC	TTAACGCTGCCCAATATCCC	55.0	50.0	57.8	58.0	1393 bp	1.0	78.6
ndhF-rpl32_p3	GAAGCGGAGTGAAGGGTATG	AAAAGCTTTTAACGCTGCCC	55.0	45.0	58.1	57.9	1404 bp	1.0	78.4
ndhF-rpl32_p4	AGCGGAGTGAAGGGTATGAT	TTAACGCTGCCCAATATCCC	50.0	50.0	58.2	58.0	1394 bp	1.0	78.2
ndhF-rpl32_p5	GCGGAGTGAAGGGTATGATC	AAAAGCTTTTAACGCTGCCC	55.0	45.0	57.8	57.9	1401 bp	1.0	77.6