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psbE-rps12

LSC · 72784-73951 · 1168 bp

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Pi 0.000856

MarkerSeek score 59.20

Primer available yes

Label psbE-rps12

Feature Metadata

label_name: psbE-rps12
feature_type: igs
region: LSC
length_bp: 1168
variable_sites: 1
pi: 0.000856
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-rps12_p1	CAACCCGCAATGAATAGGGA	CGGGGGATTTCGAGACATTT	50.0	50.0	57.9	58.0	1339 bp	1.0	79.3
psbE-rps12_p2	AGAACGTTCTCCTGTGCTTC	CGGGGGATTTCGAGACATTT	50.0	50.0	57.8	58.0	1262 bp	1.0	78.5
psbE-rps12_p3	ACGAATAACCAACCCGCAAT	CGGGGGATTTCGAGACATTT	45.0	50.0	58.2	58.0	1348 bp	1.0	78.4
psbE-rps12_p4	TGCTGACGAATAACCAACCC	CGGGGGATTTCGAGACATTT	50.0	50.0	58.2	58.0	1353 bp	1.0	78.3
psbE-rps12_p5	CGAATAACCAACCCGCAATG	CGGGGGATTTCGAGACATTT	50.0	50.0	57.8	58.0	1347 bp	1.0	78.3