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psbZ-trnG-GCC

LSC · 41156-41522 · 367 bp

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Pi 0.00272

MarkerSeek score 55.50

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 367
variable_sites: 1
pi: 0.00272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TCCCGATGGTTGGTCAAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	517 bp	1.0	76.0
psbZ-trnG-GCC_p2	TTTGCTTCTCCCGATGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.5	57.8	525 bp	1.0	75.1
psbZ-trnG-GCC_p3	CCCGATGGTTGGTCAAGTAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.5	57.8	516 bp	1.0	74.9
psbZ-trnG-GCC_p4	ATTTGCTTCTCCCGATGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.5	57.8	526 bp	1.0	74.7
psbZ-trnG-GCC_p5	CGTTGTATTTGCTTCTCCCG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.2	57.8	532 bp	1.0	72.3