Hotspot

trnT-GGU-psbD

LSC · 36525-37821 · 1297 bp

Back to Consolea

Pi 0
MarkerSeek score 58.80
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1297
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG TCCCTCCGTAACCAGTCATC 55.0 55.0 58.1 58.5 1431-1432 bp 1.0 75.4
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA TCCCTCCGTAACCAGTCATC 50.0 55.0 58.1 58.5 1434-1435 bp 1.0 75.2
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA TCCCTCCGTAACCAGTCATC 50.0 55.0 58.3 58.5 1408-1409 bp 1.0 73.6
trnT-GGU-psbD_p4 GGCCCTTTTAACTCAGTGGT TCCCTCCGTAACCAGTCATC 50.0 55.0 57.7 58.5 1446-1447 bp 1.0 73.6
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG GCAATAGACCAGACCAACCT 55.0 50.0 58.1 57.2 1466-1467 bp 1.0 73.2