Hotspot

ycf1

SSC · 124503-127901 · 3399 bp

Back to Conringia

Pi 0.033

MarkerSeek score 62.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 3399
variable_sites: 112
pi: 0.033
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTTTTACGCCTTTGGGAACG	TGATCCATTTTTGCACGGGA	50.0	45.0	58.0	58.1	627 bp	1.0	83.3
ycf1_p2	GAGTGCGCTTTTGCTTGAAA	TGATCCATTTTTGCACGGGA	45.0	45.0	58.2	58.1	663 bp	1.0	82.3
ycf1_p3	GACCACTCGGGAAGTTGAAA	TGATCCATTTTTGCACGGGA	50.0	45.0	57.8	58.1	981 bp	1.0	81.5
ycf1_p4	AGTGCGCTTTTGCTTGAAAA	TGATCCATTTTTGCACGGGA	40.0	45.0	57.7	58.1	662 bp	1.0	81.2
ycf1_p5	GCCTTTGGGAACGCATAGAT	TGATCCATTTTTGCACGGGA	50.0	45.0	58.3	58.1	620 bp	0.5	56.8