Hotspot

ndhF-rpl32

SSC · 111001-111833 · 833 bp

Back to Conringia

Pi 0.0506

MarkerSeek score 77.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 833
variable_sites: 41
pi: 0.0506
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCAATAGGTCGTGTGAACCA	ACGAATACGCTTTTTCGAGA	50.0	40.0	58.2	55.5	1393 bp	0.5	38.8
ndhF-rpl32_p2	GGTCGTGTGAACCAAAAACC	ACGAATACGCTTTTTCGAGA	50.0	40.0	57.8	55.5	1387 bp	0.5	38.8
ndhF-rpl32_p3	GCAATAGGTCGTGTGAACCA	ACTTAGTGTGGCTTATTGTGGA	50.0	40.9	58.2	57.4	1903 bp	0.5	37.4
ndhF-rpl32_p4	GGTCGTGTGAACCAAAAACC	ACTTAGTGTGGCTTATTGTGGA	50.0	40.9	57.8	57.4	1897 bp	0.5	37.3
ndhF-rpl32_p5	GCAATAGGTCGTGTGAACCA	CACTTAGTGTGGCTTATTGTGG	50.0	45.5	58.2	57.3	1904 bp	0.5	36.9