Hotspot

trnL-UUA-trnF-UUC

LSC · 46786-47282 · 497 bp

Back to Conringia

Pi 0.106

MarkerSeek score 82.20

Primer available yes

Label trnL-UUA-trnF-UUC

Feature Metadata

label_name: trnL-UUA-trnF-UUC
feature_type: igs
region: LSC
length_bp: 497
variable_sites: 48
pi: 0.106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UUA-trnF-UUC_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	587-794 bp	1.0	85.4
trnL-UUA-trnF-UUC_p2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	584-791 bp	1.0	82.1
trnL-UUA-trnF-UUC_p3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	576-783 bp	1.0	81.5
trnL-UUA-trnF-UUC_p4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGG	50.0	55.0	57.5	58.6	549-756 bp	1.0	81.4
trnL-UUA-trnF-UUC_p5	AATCGTGAGGGTTCAAGTCC	TGAACTGGTGACACGAGGAT	50.0	50.0	57.5	58.7	585-792 bp	1.0	80.5