Hotspot

atpH-atpI

LSC · 13355-13833 · 479 bp

Back to Conringia

Pi 0.0369

MarkerSeek score 76.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 479
variable_sites: 17
pi: 0.0369
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	TACAAGCGGGATTCAAGCTC	50.0	50.0	58.0	58.0	624-625 bp	1.0	84.1
atpH-atpI_p2	AACCGAAGCAGCAGAAATCA	TACAAGCGGGATTCAAGCTC	45.0	50.0	58.1	58.0	588-589 bp	1.0	83.7
atpH-atpI_p3	AATAGAAGCAAGCCCGACAG	TTTTTGCCACTTTAGCTGCG	50.0	45.0	58.0	57.9	601-602 bp	1.0	83.4
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.9	58.0	646-647 bp	1.0	83.2
atpH-atpI_p5	GCTGTACCTTGACCAACTCC	TACAAGCGGGATTCAAGCTC	55.0	50.0	58.2	58.0	650-651 bp	1.0	82.6