Hotspot

trnS-AGC-trnG-GGA

LSC · 7802-8394 · 593 bp

Back to Conringia

Pi 0.0497
MarkerSeek score 74.90
Primer available yes
Label trnS-AGC-trnG-GGA

Feature Metadata

label_name
trnS-AGC-trnG-GGA
feature_type
igs
region
LSC
length_bp
593
variable_sites
29
pi
0.0497
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-AGC-trnG-GGA_p1 GTTTGGCAAGCAGCTGTAAG TGGTTTCATTCGGCTCCTTT 50.0 45.0 57.9 57.7 1984-2021 bp 1.0 83.1
trnS-AGC-trnG-GGA_p2 GTTTGGCAAGCAGCTGTAAG TTGGTTTCATTCGGCTCCTT 50.0 45.0 57.9 57.7 1985-2022 bp 1.0 83.1
trnS-AGC-trnG-GGA_p3 GTTTGGCAAGCAGCTGTAAG TTTGGTTTCATTCGGCTCCT 50.0 45.0 57.9 57.7 1986-2023 bp 1.0 83.1
trnS-AGC-trnG-GGA_p4 CCAACGCTTTAGTCCACTCA TGGTTTCATTCGGCTCCTTT 50.0 45.0 57.8 57.7 1298 bp 0.5 56.4
trnS-AGC-trnG-GGA_p5 CCAACGCTTTAGTCCACTCA TTGGTTTCATTCGGCTCCTT 50.0 45.0 57.8 57.7 1299 bp 0.5 56.4