Hotspot

psbK-psbI

LSC · 7115-7507 · 393 bp

Back to Conringia

Pi 0.0663

MarkerSeek score 75.30

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 393
variable_sites: 23
pi: 0.0663
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCAGCTGTAAG	ACAAAAAGTTTGAGAGTAAGCA	50.0	31.8	57.9	54.4	411-446 bp	1.0	48.3
psbK-psbI_p2	TGGCAAGCAGCTGTAAGTTT	ACAAAAAGTTTGAGAGTAAGCA	45.0	31.8	58.3	54.4	408-443 bp	1.0	48.3
psbK-psbI_p3	TTGGCAAGCAGCTGTAAGTT	ACAAAAAGTTTGAGAGTAAGCA	45.0	31.8	58.3	54.4	409-444 bp	1.0	48.3
psbK-psbI_p4	TTTGGCAAGCAGCTGTAAGT	ACAAAAAGTTTGAGAGTAAGCA	45.0	31.8	58.3	54.4	410-445 bp	1.0	48.3
psbK-psbI_p5	GTTTGGCAAGCAGCTGTAAG	ACAAAAAGTTTGAGAGTAAGCAT	50.0	30.4	57.9	55.0	411-446 bp	1.0	48.3