Hotspot

ycf2

Genome · 30469-37002 · 6534 bp

Back to Conocephalum

Pi 0.0148

MarkerSeek score 49.40

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 6534
variable_sites: 95
pi: 0.0148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TGATTTCTTGAATTCTCAGTTGA	CTTCGCGAAGGGTCTGTTAA	30.4	50.0	54.2	57.9	1392-1494 bp	1.0	45.9
ycf2_p2	TGATTTCTTGAATTCTCAGTTGAA	CTTCGCGAAGGGTCTGTTAA	29.2	50.0	55.0	57.9	1392-1494 bp	1.0	45.9
ycf2_p3	ATGATTTCTTGAATTCTCAGTTGA	CTTCGCGAAGGGTCTGTTAA	29.2	50.0	54.8	57.9	1393-1495 bp	1.0	45.9
ycf2_p4	TGATTTCTTGAATTCTCAGTTGA	TTCGCGAAGGGTCTGTTAAA	30.4	45.0	54.2	57.5	1391-1493 bp	1.0	45.9
ycf2_p5	TGATTTCTTGAATTCTCAGTTG	CTTCGCGAAGGGTCTGTTAA	31.8	50.0	52.7	57.9	1392-1494 bp	1.0	45.9