Hotspot

ndhB

Genome · 1631-3649 · 2019 bp

Back to Conocephalum

Pi 0.00952

MarkerSeek score 54.30

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: Genome
length_bp: 2019
variable_sites: 19
pi: 0.00952
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	GGAGCAGCAAGAAAACGTTC	CGCGTGTTTACCATTTCACC	50.0	50.0	58.0	58.0	2489-2515 bp	1.0	81.8
ndhB_p2	GGAAAAGCGTTAGCGATTCG	CGCGTGTTTACCATTTCACC	50.0	50.0	57.9	58.0	2519-2545 bp	1.0	81.5
ndhB_p3	GGAGCAGCAAGAAAACGTTC	GACTCGAACCTCCATGCTTT	50.0	50.0	58.0	57.9	2529-2555 bp	1.0	81.3
ndhB_p4	GGAAAAGCGTTAGCGATTCG	GACTCGAACCTCCATGCTTT	50.0	50.0	57.9	57.9	2559-2585 bp	1.0	80.9
ndhB_p5	GCAGCAAGAAAACGTTCAGG	CGCGTGTTTACCATTTCACC	50.0	50.0	58.2	58.0	2486-2512 bp	1.0	80.1