Hotspot

ycf1-chlN

Genome · 108614-108760 · 147 bp

Back to Conocephalum

Pi 0.0952

MarkerSeek score 86.90

Primer available yes

Label ycf1-chlN

Feature Metadata

label_name: ycf1-chlN
feature_type: igs
region: Genome
length_bp: 147
variable_sites: 14
pi: 0.0952
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-chlN_p1	AACCCATAGCACAGAAAGCA	ACTTGAACTTGTTACACGTCCT	45.0	40.9	57.4	57.9	268-269 bp	1.0	62.4
ycf1-chlN_p2	ACCCATAGCACAGAAAGCAA	ACTTGAACTTGTTACACGTCCT	45.0	40.9	57.4	57.9	267-268 bp	1.0	62.4
ycf1-chlN_p3	AACCCATAGCACAGAAAGCA	ACTTGAACTTGTTACACGTCC	45.0	42.9	57.4	56.4	268-269 bp	1.0	58.9
ycf1-chlN_p4	ACCCATAGCACAGAAAGCAA	ACTTGAACTTGTTACACGTCC	45.0	42.9	57.4	56.4	267-268 bp	1.0	58.9
ycf1-chlN_p5	TGGAACCCATAGCACAGAAAG	ACTTGAACTTGTTACACGTCCT	47.6	40.9	57.9	57.9	271-272 bp	1.0	58.5