Hotspot

cysT-trnL-UAG

Genome · 95054-95301 · 248 bp

Back to Conocephalum

Pi 0.0833

MarkerSeek score 79.50

Primer available yes

Label cysT-trnL-UAG

Feature Metadata

label_name: cysT-trnL-UAG
feature_type: igs
region: Genome
length_bp: 248
variable_sites: 20
pi: 0.0833
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cysT-trnL-UAG_p1	TGAACAATATGATTATCAAAGTGCT	AGCAGCGTGTCTACCAATTT	28.0	45.0	55.4	57.8	391-395 bp	1.0	45.7
cysT-trnL-UAG_p2	TGAACAATATGATTATCAAAGTGCT	TCCTAAGAGCAGCGTGTCTA	28.0	50.0	55.4	57.9	398-402 bp	1.0	45.6
cysT-trnL-UAG_p3	TGAACAATATGATTATCAAAGTGCT	CTAAGAGCAGCGTGTCTACC	28.0	55.0	55.4	57.8	396-400 bp	1.0	45.6
cysT-trnL-UAG_p4	TGAACAATATGATTATCAAAGTGCT	TCGAACCGAGATGCATTAGC	28.0	50.0	55.4	58.1	424-428 bp	1.0	45.3
cysT-trnL-UAG_p5	TGAACAATATGATTATCAAAGTGCT	GGATTCGAACCGAGATGCAT	28.0	50.0	55.4	58.1	428-432 bp	1.0	45.2