Hotspot

rpl16-rps3

Genome · 77989-78051 · 63 bp

Back to Conocephalum

Pi 0.098

MarkerSeek score 73.10

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: Genome
length_bp: 63
variable_sites: 5
pi: 0.098
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AACGAGTCACACACTAAGCA	AGTTTGGATATTTCAAGATGAAT	45.0	26.1	57.1	52.1	100-112 bp	1.0	47.8
rpl16-rps3_p2	ACGAGTCACACACTAAGCAT	AAGTTTGGATATTTCAAGATGAA	45.0	26.1	56.9	52.3	100-112 bp	1.0	47.8
rpl16-rps3_p3	AACGAGTCACACACTAAGCA	AAAGTTTGGATATTTCAAGATGA	45.0	26.1	57.1	52.3	102-114 bp	1.0	47.7
rpl16-rps3_p4	AACGAGTCACACACTAAGCA	AAGTTTGGATATTTCAAGATGAA	45.0	26.1	57.1	52.3	101-113 bp	1.0	47.7
rpl16-rps3_p5	AACGAGTCACACACTAAGCA	AAAGTTTGGATATTTCAAGATGAA	45.0	25.0	57.1	53.2	102-114 bp	1.0	47.7