Hotspot

trnT-UGU-trnL-UAA

Genome · 50828-51028 · 201 bp

Back to Conocephalum

Pi 0.05
MarkerSeek score 71.40
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
Genome
length_bp
201
variable_sites
10
pi
0.05
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GACCTCTGAGCTAAACAGGC TTTAAGTCCGCAGCGTCTAC 55.0 50.0 58.0 58.0 259-260 bp 1.0 83.1
trnT-UGU-trnL-UAA_p2 GACCTCTGAGCTAAACAGGC AATTTAAGTCCGCAGCGTCT 55.0 45.0 58.0 57.9 261-262 bp 1.0 82.5
trnT-UGU-trnL-UAA_p3 GAGTCGAACCGATGACCATC TTTAAGTCCGCAGCGTCTAC 55.0 50.0 58.2 58.0 300-301 bp 1.0 81.6
trnT-UGU-trnL-UAA_p4 GAGTCGAACCGATGACCATC AATTTAAGTCCGCAGCGTCT 55.0 45.0 58.2 57.9 302-303 bp 1.0 80.9
trnT-UGU-trnL-UAA_p5 GACCTCTGAGCTAAACAGGC TACCAATTTCGCCATACCCC 55.0 50.0 58.0 57.6 242-243 bp 1.0 80.7