Hotspot

trnS-UGA-rps4

Genome · 49714-49939 · 226 bp

Back to Conocephalum

Pi 0.0543
MarkerSeek score 72.60
Primer available yes
Label trnS-UGA-rps4

Feature Metadata

label_name
trnS-UGA-rps4
feature_type
igs
region
Genome
length_bp
226
variable_sites
12
pi
0.0543
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-rps4_p1 TGGTTGAGTGGTTTAAGGCA TGTAGAATATTACTCTCGTCAAGT 45.0 33.3 57.3 54.9 334-390 bp 1.0 43.8
trnS-UGA-rps4_p2 TGGTTGAGTGGTTTAAGGCA AGTTGTAGAATATTACTCTCGTCAAGT 45.0 33.3 57.3 58.0 337-393 bp 1.0 43.7
trnS-UGA-rps4_p3 TGGTTGAGTGGTTTAAGGCA AGTTGTAGAATATTACTCTCGTCA 45.0 33.3 57.3 54.9 337-393 bp 1.0 43.7
trnS-UGA-rps4_p4 TGGTTGAGTGGTTTAAGGCA GTTGTAGAATATTACTCTCGTCAAGT 45.0 34.6 57.3 56.9 336-392 bp 1.0 43.7
trnS-UGA-rps4_p5 TGGTTGAGTGGTTTAAGGCA AGTTGTAGAATATTACTCTCGTCAAG 45.0 34.6 57.3 56.6 337-393 bp 1.0 43.7