Hotspot

psbA-trnH-GUG

Genome · 29622-30060 · 439 bp

Back to Conocephalum

Pi 0.0822

MarkerSeek score 89.10

Primer available yes

Label psbA-trnH-GUG

Feature Metadata

label_name: psbA-trnH-GUG
feature_type: igs
region: Genome
length_bp: 439
variable_sites: 35
pi: 0.0822
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 89.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnH-GUG_p1	GCTCCTGCTGTAAACGGTTA	CTGCCTTAATCCACTTGGCT	50.0	50.0	57.9	57.9	475-487 bp	1.0	86.2
psbA-trnH-GUG_p2	GCTCCTGCTGTAAACGGTTA	GATCCACAATCCACTGCCTT	50.0	50.0	57.9	57.9	488-500 bp	1.0	86.0
psbA-trnH-GUG_p3	GCTCCTGCTGTAAACGGTTA	ACTGCCTTAATCCACTTGGC	50.0	50.0	57.9	58.2	476-488 bp	1.0	86.0
psbA-trnH-GUG_p4	GCTCCTGCTGTAAACGGTTA	TTATATTGGGCGAACGACGG	50.0	50.0	57.9	58.1	529-541 bp	1.0	85.3
psbA-trnH-GUG_p5	GTAACGCTCACAACTTCCCT	CTGCCTTAATCCACTTGGCT	50.0	50.0	57.8	57.9	516-528 bp	1.0	85.0