Hotspot

psaM-trnS-GCU

Genome · 22640-23022 · 383 bp

Back to Conocephalum

Pi 0.135

MarkerSeek score 87.70

Primer available yes

Label psaM-trnS-GCU

Feature Metadata

label_name: psaM-trnS-GCU
feature_type: igs
region: Genome
length_bp: 383
variable_sites: 51
pi: 0.135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 87.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaM-trnS-GCU_p1	TGACCTCAATTTCAGATAGTCA	AACCCTCGGTACAAAAAGCT	36.4	45.0	54.6	57.3	551 bp	1.0	48.1
psaM-trnS-GCU_p2	TGACCTCAATTTCAGATAGTCA	ACCCTCGGTACAAAAAGCTT	36.4	45.0	54.6	57.3	550 bp	1.0	48.1
psaM-trnS-GCU_p3	TGACCTCAATTTCAGATAGTCA	CGGAGAGAGAGGGATTCGAA	36.4	55.0	54.6	58.3	569 bp	1.0	47.9
psaM-trnS-GCU_p4	TGACCTCAATTTCAGATAGTCAA	CGGAGAGAGAGGGATTCGAA	34.8	55.0	55.3	58.3	569 bp	1.0	47.9
psaM-trnS-GCU_p5	TGACCTCAATTTCAGATAGTCA	AACGGAGAGAGAGGGATTCG	36.4	55.0	54.6	58.6	571 bp	1.0	47.8