Hotspot

trnR-UCU-trnG-GCC

Genome · 21306-21387 · 82 bp

Back to Conocephalum

Pi 0.061

MarkerSeek score 72.60

Primer available yes

Label trnR-UCU-trnG-GCC

Feature Metadata

label_name: trnR-UCU-trnG-GCC
feature_type: igs
region: Genome
length_bp: 82
variable_sites: 5
pi: 0.061
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnG-GCC_p1	CCTCTGTCCTATCCTTTAGACG	ACCCTTAGCCTTCCAAGCTA	50.0	50.0	57.2	58.0	161-162 bp	1.0	59.5
trnR-UCU-trnG-GCC_p2	CCTCTGTCCTATCCTTTAGACG	TTCCAAGCTAATGATGCGGG	50.0	50.0	57.2	58.3	151-152 bp	1.0	57.4
trnR-UCU-trnG-GCC_p3	GTCCTATCCTTTAGACGATGGAC	ACCCTTAGCCTTCCAAGCTA	47.8	50.0	57.9	58.0	156-157 bp	1.0	57.3
trnR-UCU-trnG-GCC_p4	CTGTCCTATCCTTTAGACGATGG	ACCCTTAGCCTTCCAAGCTA	47.8	50.0	57.9	58.0	158-159 bp	1.0	57.2
trnR-UCU-trnG-GCC_p5	TGTCCTATCCTTTAGACGATGGA	ACCCTTAGCCTTCCAAGCTA	43.5	50.0	58.2	58.0	157-158 bp	1.0	56.7