Hotspot

atpI-atpH

Genome · 17640-18052 · 413 bp

Back to Conocephalum

Pi 0.0545

MarkerSeek score 74.90

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: Genome
length_bp: 413
variable_sites: 22
pi: 0.0545
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TTTTTGCTACACTTGCAGCA	GCAGCAATAACAGAAGCAGC	40.0	50.0	56.8	58.1	507-510 bp	1.0	74.2
atpI-atpH_p2	TTTTTGCTACACTTGCAGCA	AAGCAGCAGAAATCAAGGGG	40.0	50.0	56.8	58.4	494-497 bp	1.0	71.5
atpI-atpH_p3	TTTTTGCTACACTTGCAGCA	CCAGCAGCAATAACAGAAGC	40.0	50.0	56.8	57.4	510-513 bp	1.0	70.4
atpI-atpH_p4	ATTTTTGCTACACTTGCAGCA	GCAGCAATAACAGAAGCAGC	38.1	50.0	57.3	58.1	508-511 bp	1.0	70.3
atpI-atpH_p5	TTTTTGCTACACTTGCAGCA	GCAGCAGAAATCAAGGGGTT	40.0	50.0	56.8	58.7	492-495 bp	1.0	69.2