Hotspot

rps7-ndhB

Genome · 1429-1630 · 202 bp

Back to Conocephalum

Pi 0.154

MarkerSeek score 80.30

Primer available yes

Label rps7-ndhB

Feature Metadata

label_name: rps7-ndhB
feature_type: igs
region: Genome
length_bp: 202
variable_sites: 31
pi: 0.154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-ndhB_p1	TGATGCAGCTAGAGATAATGGG	AGATGCATTCTGGTAAAATAGT	45.5	31.8	57.5	53.2	356-357 bp	1.0	47.2
rps7-ndhB_p2	TGCAGCTAGAGATAATGGGA	AGATGCATTCTGGTAAAATAGT	45.0	31.8	55.4	53.2	353-354 bp	1.0	47.2
rps7-ndhB_p3	TGATGCAGCTAGAGATAATGGG	AAGATGCATTCTGGTAAAATAGT	45.5	30.4	57.5	54.1	357-358 bp	1.0	47.2
rps7-ndhB_p4	TGCAGCTAGAGATAATGGGA	AAGATGCATTCTGGTAAAATAGT	45.0	30.4	55.4	54.1	354-355 bp	1.0	47.2
rps7-ndhB_p5	GATGCAGCTAGAGATAATGGGA	AGATGCATTCTGGTAAAATAGT	45.5	31.8	57.2	53.2	355-356 bp	1.0	47.2