Hotspot

ycf2

LSC · 87713-93985 · 6273 bp

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Pi 0.00499

MarkerSeek score 37.50

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: LSC
length_bp: 6273
variable_sites: 95
pi: 0.00499
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TACCTAGCGACAAACTCCCA	GTTCCAGCTCCGTATGAAGG	50.0	55.0	58.1	58.1	178-268 bp	1.0	87.3
ycf2_p2	TACCTAGCGACAAACTCCCA	CAGCTCCGTATGAAGGTCAC	50.0	55.0	58.1	58.1	174-264 bp	1.0	87.3
ycf2_p3	CGACAAACTCCCATGTTCCT	GTTCCAGCTCCGTATGAAGG	50.0	55.0	57.8	58.1	171-261 bp	1.0	86.6
ycf2_p4	CGACAAACTCCCATGTTCCT	CAGCTCCGTATGAAGGTCAC	50.0	55.0	57.8	58.1	167-257 bp	1.0	86.6
ycf2_p5	TACCTAGCGACAAACTCCCA	TAGCAGTTCCAGCTCCGTAT	50.0	50.0	58.1	58.2	183-273 bp	1.0	85.9