Hotspot

ccsA

SSC · 116452-117465 · 1014 bp

Back to Conioselinum

Pi 0.00391

MarkerSeek score 39.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 1014
variable_sites: 12
pi: 0.00391
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	TGGACCGCGAGAGTTATTTG	50.0	50.0	58.0	58.0	1498-1505 bp	1.0	81.9
ccsA_p2	AACCGACAAAAGCCTGGAAT	TGGACCGCGAGAGTTATTTG	45.0	50.0	58.0	58.0	1918-1925 bp	1.0	81.8
ccsA_p3	ACCGACAAAAGCCTGGAATT	TGGACCGCGAGAGTTATTTG	45.0	50.0	58.0	58.0	1917-1924 bp	1.0	81.8
ccsA_p4	AACCGACAAAAGCCTGGAAT	GGACCGCGAGAGTTATTTGT	45.0	50.0	58.0	58.0	1917-1924 bp	1.0	81.8
ccsA_p5	ACCGACAAAAGCCTGGAATT	GGACCGCGAGAGTTATTTGT	45.0	50.0	58.0	58.0	1916-1923 bp	1.0	81.8