Hotspot

ndhF-rpl32

SSC · 114121-115176 · 1056 bp

Back to Conioselinum

Pi 0.00968
MarkerSeek score 54.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1056
variable_sites
31
pi
0.00968
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGCCGACGGAAAAAGAAGAA TCCAAATATTTTTACGAATACGCT 45.0 29.2 58.0 55.3 1181-1221 bp 1.0 45.2
ndhF-rpl32_p2 AAAAGAAGAAGTCCCGCTCC TCCAAATATTTTTACGAATACGCT 50.0 29.2 57.8 55.3 1171-1211 bp 1.0 45.2
ndhF-rpl32_p3 GTTGCCGACGGAAAAAGAAG TCCAAATATTTTTACGAATACGCT 50.0 29.2 58.0 55.3 1183-1223 bp 0.909091 41.0
ndhF-rpl32_p4 TTGCCGACGGAAAAAGAAGA TCCAAATATTTTTACGAATACGCT 45.0 29.2 58.0 55.3 1182-1222 bp 0.909091 41.0
ndhF-rpl32_p5 TTTGTTGCCGACGGAAAAAG TCCAAATATTTTTACGAATACGCT 45.0 29.2 57.8 55.3 1186-1226 bp 0.909091 41.0