Hotspot

ycf1-ndhF

SSC · 111835-111873 · 39 bp

Back to Conioselinum

Pi 0.0742

MarkerSeek score 73.10

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 39
variable_sites: 7
pi: 0.0742
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACCGAATTGGAATTCCTGTCT	GTGGGCGCATCTCTTCTTAT	42.9	50.0	57.3	57.8	142-180 bp	1.0	72.4
ycf1-ndhF_p2	ACCGAATTGGAATTCCTGTCT	TGGGCGCATCTCTTCTTATC	42.9	50.0	57.3	57.5	141-179 bp	1.0	70.1
ycf1-ndhF_p3	ACCGAATTGGAATTCCTGTC	GTGGGCGCATCTCTTCTTAT	45.0	50.0	55.7	57.8	142-180 bp	1.0	68.0
ycf1-ndhF_p4	ACCGAATTGGAATTCCTGTCT	AGTGGGCGCATCTCTTCTTA	42.9	50.0	57.3	58.8	143-181 bp	1.0	67.9
ycf1-ndhF_p5	ACCGAATTGGAATTCCTGTCT	TAGTGGGCGCATCTCTTCTT	42.9	50.0	57.3	58.8	144-182 bp	1.0	67.9