Hotspot

rpl20-rps12

LSC · 70178-70963 · 786 bp

Back to Conioselinum

Pi 0.00493

MarkerSeek score 52.80

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 786
variable_sites: 13
pi: 0.00493
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATTTCCCCGT	CAGCGTCGAGGAACATGTAC	50.0	55.0	58.0	58.7	828-850 bp	1.0	77.7
rpl20-rps12_p2	TCCGAGCTATATTTCCCCGT	AGCGTCGAGGAACATGTACT	50.0	50.0	58.0	58.8	827-849 bp	1.0	76.9
rpl20-rps12_p3	TCCGAGCTATATTTCCCCGT	TCAGCGTCGAGGAACATGTA	50.0	50.0	58.0	58.8	829-851 bp	1.0	76.9
rpl20-rps12_p4	TCCGAGCTATATTTCCCCGT	GCGTCGAGGAACATGTACTA	50.0	50.0	58.0	57.2	826-848 bp	1.0	76.8
rpl20-rps12_p5	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	882-904 bp	1.0	76.6